Image Challenge 22 - Identify the object

May 11, 201013comments

A laboratory received a diarrhoeal stool specimen from a 4 years old kid complaining of abdominal cramps and diarrhoea. Upon concentration of stool by formal-ether method and staining by modified AFB technique, the following objects measuring about 4 microns diameter were observed under oil immersion objective.

What is your diagnosis? and based on what criteria?

Enter your answers in comments section and first 3 correct entries will receive surprise gifts

And the answer is here

Image Courtesy: DPDx

Related Posts: Other Image Challenges
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+ comments + 13 comments

May 12, 2010 at 12:00 AM

Is it a cyclospora sps or cryptosporidium sps.....

May 12, 2010 at 9:11 AM

Crytosporidium parvum

May 12, 2010 at 9:12 AM

Cryptosporidium parvum

May 12, 2010 at 9:14 AM

It is Cryptosporidium parvum. Based on clinical complaint and the staining method and morphology of the organism. MAFS technique is used for Crypto, Isospora etc.

May 12, 2010 at 10:14 AM

i gueess blood cell

May 13, 2010 at 9:39 PM

Well, given the Hx of the patient and the Diagnostic modality (Modified AFB of fecal specimen) the DDs should be restricted to Cryptosporidium parvum and Cyclospora. Cyclospora is ruled out as diameter of the oocyst is around 8 -10 micron meters and here the diameter is 4 micron meters. The diameter of the oocyst of Cryptosporidium parvum is b/w 4 - 5 micron meters. Isospora belli is ruled out coz of shape of oocyst, which in this case is oval.

Final dx : Infx with Cryptosporidium parvum.

Object : OOcyst of Cryptosporidium parvum.

May 15, 2010 at 8:55 AM

Cryptosporidium parvum/C. hominis infection is my diagnosis as opposed to Cyclospora as the oocysts size is 4um and not greater (cyclospora oocysts are usually is 8-10um). Also Cryptosporidium stain red in modified AFB while cyclospora could be colorless to pink or red. UV light can be used as Cyclospora oocysts show autofluorescence...

May 16, 2010 at 4:28 AM

Cryptosporidium parvum

May 16, 2010 at 1:15 PM

It is the oocyst of cryptosporidium which is one of the commonest organisms causing diarrhoea and is usually stained by modified acidfast stain and is usually of the size of 4-6 microns.

May 17, 2010 at 3:32 PM

Mostly crypto - Children are more prone for this!

EM Shankar
May 17, 2010 at 6:03 PM

Cryptosporidium parvum???? Or Cyclospora cayetanensis

May 29, 2010 at 5:52 PM

Image is suggestive of an Oocyst of Cryptosporidium parvum.
-Subhash Angadi.

Dr. Kiran
June 16, 2010 at 8:03 PM

Cryptosporidium parvum

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